Validity of using 2,4-3H-labeled bile acids to study bile acid kinetics in man
LaRusso, N.F.; Hoffman, N.E.; Hofmann, A.F.
Journal of Laboratory and Clinical Medicine 84(5): 759-765
1974
ISSN/ISBN: 0022-2143 PMID: 4283799 Document Number: 73163
Chenodeoxycholic acid-2,2',4,4'-3H was given simultaneously with the -24-14C acid. A similar study was done with labelled cholic acid. Estimation of bile acid kinetics in healthy subjects was valid with chenodeoxycholic acid-3H but there was an error greater than 10% with cholic acid-3H. The 2,2',4,4'-3H label was unsatisfactory for tracing the complete metabolic fate of the steroid moiety of bile acids because 3H is removed by bacteria during intestinal passage.