Characterization of Extraintestinal Pathogenic Escherichia Coli From MEAT IN SOUTHERN THAILAND
Sukkua, K.; Pomwised, R.; Rattanachuay, P.; Khianngam, S.; Sukhumungoon, P.
Southeast Asian Journal of Tropical Medicine and Public Health 48(1): 98-108
2017
ISSN/ISBN: 0125-1562 PMID: 29644826 Document Number: 693990
Extraintestinal pathogenic Escherichia coli (ExPEC) is an E. coli group, which causes diseases in systems outside human intestinal tract. ExPEC isolates were recovered from fresh chicken (25%) and pork (10%) meats, but not beef and shrimp, from markets in southern Thailand. Among the 14 ExPEC strains isolated, all carried iutA and fimH, coding for aerobactin and type 1 fimbriae, respectively. Two ExPEC strains from chicken meat possessed kpsMTK1 coding for K1 capsular antigen, responsible for neonatal meningitis. Antimicrobial susceptibility assay. revealed that all ExPEC were resistant to streptomycin and carried bla(TEM), but susceptible to imipenem. Phylogenetic group analysis showed that 4, 4, and 6 ExPEC strains belonged to group A, B1 and D, respectively. ExPEC strains were classified into four serotypes, namely, 08 (2 strains), 015 (2 strains), 025 (1 strain), and 0127a (1 strain), with the remaining untypeable. DNA profiling analysis by BOX-PCR revealed clonality of strains with the same serotype. The existence of ExPEC in meat products should cause concern regarding food safety and public health not only in southern Thailand but also throughout the country.