Chinese medicinal compound CFF-1 induces the apoptosis and cycle-arrest of prostate cancer cells via the PI3K/AKT/FOXO1 signaling pathway
Zhang, Y.; Wu, Z.-M.; Lei, B.-H.; Lu, Z.-J.; Zhu, Q.-Y.; Xu, F.-S.; Zhang, M.-S.; Liu, P.
Zhonghua Nan Ke Xue 23(9): 828-837
2017
ISSN/ISBN: 1009-3591 PMID: 29726666 Document Number: 693946
目的: 研究复方中药CFF-1诱导前列腺癌细胞的凋亡作用及其相关分子机制的探讨。方法: 通过形态学观察、噻唑蓝（MTT）比色实验、CCK-8实验测定前列腺癌细胞的存活能力；采用DAPI染色法测定细胞核凝缩破裂；运用AnnexinV-FITC/PI双染流式细胞术测定前列腺癌细胞的凋亡率。再通过PI单染流式细胞术测定前列腺癌细胞的周期变化；以及采用蛋白质免疫印迹实验检测PI3K/AKT/FOXO1信号通路以及其下游凋亡相关蛋白和周期相关蛋白的表达。结果: 复方中药CFF-1使前列腺癌细胞周期阻滞在G1期，并呈浓度依赖性降低细胞的存活能力、增加细胞核凝缩破裂以及核小体的形成，显著提高前列腺癌细胞的凋亡率。在分子机制上，CFF-1呈现浓度依赖性下调PI3K/AKT活性，降低FOXO1磷酸化水平，进而上调FOXO1的转录活性，最终影响凋亡相关基因和周期相关基因的表达。结论: CFF-1对前列腺癌细胞的生长有显著的抑制作用，并且通过PI3K/AKT/FOXO1信号通路诱导前列腺癌细胞周期阻滞并发生凋亡，对治疗前列腺癌具有潜在的临床应用价值。.
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