Effect of transfected EphB4 on osteogenic differentiation in stem cells from human exfoliated deciduous teeth
Li, X.-M.; Yuan, C.-Y.; Zhu, S.-Y.; Liu, Z.-X.; Wang, P.-L.
Shanghai Kou Qiang Yi Xue 26(4): 399-403
2017
ISSN/ISBN: 1006-7248 PMID: 29199334 Document Number: 693691
To evaluate the effect of stem cells from human exfoliated deciduous teeth (SHED) transfected with EphB4 gene in regulating osteogenic differentiation. Human dental pulp tissue were harvested from extracted deciduous teeth and digested by collagenase and dispase. The SHEDs were transfected with transgenic (hEphB4-GFP) vector or empty vector (GFP-vector). Real time-polymerase chain reaction（real time-PCR） analysis and Western blot were used to detect the expression of EphB4 in SHEDs after transfection. EphB4-SHEDs and GFP-SHEDs were subjected to osteogenic induction and assessed by alkaline phosphatase(ALP) assay and Alizarin-red S staining. SPSS 16.0 software package was used for statistical analysis. Real time-PCR revealed that the expression of EphB4 was significantly enhanced in EphB4-SHEDs compared to GFP-SHEDs (P<0.05). The expression of EphB4 protein was significantly higher (P<0.05) in EphB4-SHEDs compared to GFP-SHEDs. ALP assay and Alizarin-red S staining demonstrated higher ALP activity and increased mineralization with EphB4-SHEDs. The results indicate that transgenic expression of EphB4 in SHEDs could promote osteogenic differentiation.