Gene polymorphism of xenobiotic detoxification in children with bronchial asthma
Lytvynets, L.Y.
Wiadomosci Lekarskie 70(3 Part 2): 558-560
2017
ISSN/ISBN: 0043-5147 PMID: 28713081 Document Number: 693139
Children are the most susceptible to harmful external factors due to the incomplete development of all functional body systems. The ecological situation the problem of influence of genetic and environmental factors on the pathogenesis of chronic respiratory diseases in children and its investigation is very impotent today. Among it the bronchial asthma is a leader among bronchial and pulmonary diseases caused by the influence of pneumotropic pollutants. The aim of our research was to study the phenotypic characteristics of asthma in Pre-Carpathian children with different types of allelic gene polymorphism of the first phase of xenobiotic detoxification of microsomal epoxide hydrolases (mEPHX1). The phenotypic peculiarities of bronchial asthma in 94 children from Ivano-Frankivsk region with different types of allelic gene polymorphism of the first phase of xenobiotic detoxification - mEPXH1 have been investigated. The material of genetic investigations was DNA isolated from leukocytes of patients' peripheral blood. Isolation and purification of DNA was carried out using commercial kits DIAtom TM DNA Prep200, GenePak DNA PCR test (LLC "Laboratory IzoGen") according to the manufacturer's recommendations. The association between polymorphism of gene mEPXH1 and susceptibility to occurrence and severity of BA in children have been described. It has been established that the manifestation of asthma as eco-determined pathology is affected by individual types of metabolic activity of genes of xenobiotic detoxification. It has been proved that patients with uncontrolled asthma have genotypes CC and GG (p <0.05) much more often. Children with asthma often have high enzymatic activity of a gene mEPHX1 (polymorphic variants of AG and AA-TT-TT). A "slow" functional state of gene mEPHX1 corresponding to homo- and hetero-zygotes for the mutant alleles: СС-АА and ТС-АА is also a frequent phenomenon. It has been established that bronchial asthma as eco-determined pathology is affected by individual variants of metabolic activity of genes of xenobiotic detoxification.