Expression of IL-34 in chronic periapical lesions and its clinical significance
Ma, N.; Qu, L.; Xu, L.-y.; Yu, Y.-q.; Qiu, L.-h.
Shanghai Kou Qiang Yi Xue 25(1): 53-57
2016
ISSN/ISBN: 1006-7248 PMID: 27063309 Document Number: 690246
To observe the expression of IL-34 mRNA in chronic periapical lesions and healthy periodontal ligaments and discuss the role of IL-34 in the etiology of chronic apical periodontitis. A total of 25 periapical tissues from chronic apical periodontitis and 22 normal periodontal ligament tissue from extracted healthy teeth for orthodontic reason were selected. The expression of IL-34mRNA was detected by real-time PCR; the expression of IL-34 protein was detected by immunohistochemical analysis. Statistical analysis was performed using SPSS 13.0 software package. The level of IL-34 mRNA expression in periapical lesions (3.53±3.07) was significantly higher than that of the normal control (1.07±0.76); IL-34 was positively expressed in lymphocytes, plasma cells and macrophages. Image analysis software indicated that the level of IL-34 protein was significantly higher in periapical lesions than that in normal control (P<0.01). IL-34 may be closely related to inflammation of chronic apical periodontitis.