A new case report of urinary tract infection due to KPC-3-producing klebsiella pneumoniae (ST258) in Spain
Sorlózano-Puerto, A.; Esteva-Fernández, D.; Oteo-Iglesias, J.; Navarro-Marí, J.M.; Gutiérrez-Fernández, J.
Archivos Espanoles de Urologia 69(7): 437-440
2016
ISSN/ISBN: 0004-0614 PMID: 27617554 Document Number: 688884
We describe the characterization of a new isolated in Spain of Klebsiella pneumoniae ST258 producing KPC-3, carbapenems non-susceptible, recovered from a sample of urine from a patient with urinary tract infection and no history of carbapenems exposure. After the isolation, identification of K. pneumoniae was performed by biochemical tests and mass spectrometry. The carbapenems susceptibility testing was performed by microdilution and E-test in cation-adjusted Mueller-Hinton. The study was completed by Rapidec® Carba NP. In order to determine the genetic basis of resistance to carbapenems we used Xpert® Carba-R for carbapenemase type and subtype was subsequently analyzed by amplification by PCR and sequencing. We demonstrated by MLST that the strain belonged to the clone of high-risk ST258. This is the first characterization, in our media, of a clinical isolated of K. pneumoniae ST258 producing KPC-3 and no history of carbapenems exposure.