Study of 1550nm Erbium Glass Laser Fractional non-ablative treatment of photoaging: Comparative clinical effects, histopathology, electron microscopy and immunohistochemistry
de Sica, R.Celli.Patriota.; Rodrigues, C.J.; Maria, D.Augusto.; Cucé, Lís.Carlos.
Journal of Cosmetic and Laser Therapy 2016: 1-36
2016
ISSN/ISBN: 1476-4172 PMID: 27223411 Document Number: 686515
Non-ablative fractional lasers have been effectively used in skin rejuvenation. Evaluate the efficacy of 1550 nm Erbium Glass Laser for facial rejuvenation through the correlation of clinical evaluation and histopathology, immunohistochemistry and electron microscopy analysis. Fifteen subjects (average age 56.4 years old, skin types II-III) with mild to moderate photodamage were submitted to biopsies and 3 facial treatments. Data from the photo assessments and the wrinkles improvement were analyzed 4 months after the treatments. The biopsy skins were fixed in neutral buffered formalin before paraffin embedding, and hematoxylin and eosin stained. The histomorphometric quantification of collagen and elastic fibers; intercellular adhesion molecule 1 expression by immunohistochemistry; and analysis of cell cycle phases, the electrical potential of the mitochondrial and IL- 1, CD34, TGF-β and caspase-3 expression by flow cytometry were analyzed. After 4 months of treatment, collagen fibers had increased by 6.68%, and ICAM-1 by 4.47% in vessel area. Significantly enhanced IL-1 and TGF-β receptor expression were identified after treatment. Proliferative responses and non-apoptosis-dependent caspase-3 activity were both observed in the cell after dermal treatment. The histopathology, immunohistochemistry and electron microscopy showed an improvement compatible to the clinical effectiveness after 4 months.