Modification of Urine Proteome in Healthy Human during 21-Day Bed Rest
Pastushkova, L.K.; Custaud, M.A.; Kononikhin, A.S.; Brzhozovsky, A.G.; Dmitrieva, L.E.; Dobrokhotov, I.V.; Tiis, E.S.; Larina, I.M.
Aviakosmicheskaia i Ekologicheskaia Meditsina 49(5): 11-16
2015
ISSN/ISBN: 0233-528X PMID: 26803880 Document Number: 681242
Mass spectromy-based proteomics was employed to analyze urine from 8 normal volunteers for a 21-day bedrest study (BR). The analysis included trypsinolysis in solution prior to liquid chromatography and tandem mass spectrometry (LC-MS/MS), and spectra processing using the bioinformatics tools. Relying on 221 IPI-indices with Score from 24 to 1700, 169 different proteins were identified. Molecular functions, biological processes and cell components as the loci of certain protein functioning were determined with the help of UniProt-GOA. Associative interactions networks were constructed using BiNGO. There were 14 proteins identified that are functional in the cardiovascular system mostly. They were annotated and -dynamics of their occurrence throughout the experiment was considered. Grounding on the biological functions of these proteins, an assumption of eligible activation of different biological processes during BR was made.