Falsely Increased HbA 1c Values by HPLC and Falsely Decreased Values by Immunoassay Lead to Identification of Hb Okayama and Help in the Management of a Diabetic Patient

Frers, C.R.; Dorn, S.; Schmidt, W.; Kochhan, L.; Simon, J.-Schultz; Arndt, R.

Clinical Laboratory 46(11-12): 569-573

2000


ISSN/ISBN: 1433-6510
PMID: 11109504
Document Number: 6795
A falsely increased HbA 1c value of 47.1% was determined by cation exchange chromatography during a routine HbA 1c measurement of the blood sample from a 63-year-old diabetic male patient living in Hamburg, Germany. In former determinations by immunological assays falsely decreased values in the range of 5.0 to 5.5% were obtained. The sample was inconspicuous in alkaline hemoglobin electrophoresis. But acid hemoglobin electrophoresis confirmed the falsely increased value. These facts let us consider the existence of a heterozygous "silent hemoglobin variant", such as hemoglobin Okayama, with an amino acid substitution in one of the first four amino acids of the beta chain, representing the epitope of common immunoassays. DNA analyses confirmed this presumption and we found the heterozygous mutation hemoglobin Okayama [ß2 (NA 2) His (CAC) → Gln (CAA)]. Knowing that an immunological assay only detects about half of the present HbA 1c , the obtained values can be used for therapeutic management of this diabetic patient.

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Falsely Increased HbA 1c Values by HPLC and Falsely Decreased Values by Immunoassay Lead to Identification of Hb Okayama and Help in the Management of a Diabetic Patient