Effect of Calcium and Iron Ions on the Level of Diesel Fuel Utilization by Actinobacteria Strains
Nogina, T.M.; Dumanskaya, T.U.; Homenko, L.A.; Kisten, A.G.
Mikrobiolohichnyi Zhurnal 77(3): 47-52
2015
ISSN/ISBN: 0201-8462 PMID: 26214898 Document Number: 679219
It has been established that the presence in the medium Ca2+ and Fe2+ ions in 1.2 - 1.3 times increases the assimilation of hydrocarbon by actinobacteria. The possibility of joint growth of binary actinobacteria cultures on mineral medium with diesel fuel without reducing the assimilation of this substrate compared to monocultures it was shown. In the conditions of co-batch culture Rhodococcus erythropolis IMV B-7277 and Dietzia maris IMV B-7278 strains in the laboratory fermenter "Biotec" (stirring speed 500 r.p.m., degree of aeration 0.3 1/1 per min, sulfite number 1.5 g O2 /1 h,) on the medium with 1.0% diesel oil in the presence of 4 mg/l Ca2+, Fe2+ and 1 g/l yeast extract for 30 hours the substrate assimilation rate was 87.8% and the concentration of biomass - 2.0 g/l.