Genome amplification and sequence analysis of human bocavirus 2
Cui, H.-D.; Jin, Y.; Xie, G.-C.; Cheng, W.-X.; Duan, Z.-J.
Bing du Xue Bao 30(3): 257-262
2014
ISSN/ISBN: 1000-8721 PMID: 25118380 Document Number: 677239
To obtain the genome sequence of human bocavirus 2 (HBoV2), different regions of HBoV2 genome were amplified through PCR in fecal specimens which had been identified as single-positive for HBoV2 in 2010. A genome sequence of HBoV2 (HBoV2-NC, 5444 bp) was obtained after sequence assembly. The phylogenetic analysis showed that HBoV2-NC had the closest evolutionary relationship with HBoV2 Lanzhou strain. The predication of inverted terminal repeats of HBoV2-NC by DINAMelt showed that inverted terminal repeats were contained in HBoV2-NC 5' terminal, which had the typical stem-loop structure in other parvoviruses. Finally, some flanking sequences of HBoV2-NC were amplified by linker-PCR.