Expression of apoptosis markers in adhesions in the abdominal cavity under the experimental conditions
Shurygina, N.A.; Shurygin, M.G.; Aiushinova, N.I.
Vestnik Rossiiskoi Akademii Meditsinskikh Nauk 2014(5-6): 29-33
2014
ISSN/ISBN: 0869-6047 PMID: 25558677 Document Number: 673921
Our aim was to study the expression of markers of caspase-dependent and caspase-independent apoptosis pathway activation in the process of repair in case of damage of the serous membrane of the abdominal cavity. On the experimental model of adhesions in the abdominal cavity (male rats Wistar, n = 40) in dynamics from 2 hours to 30 days after injury to the peritoneum studied marker expression pro-apoptosis (Bcl-x) and anti-apoptosis (Bcl-2), and PARP-1. It was found that in the conditions of traumatic injury of the peritoneum apoptosis and anti-apoptosis occur in parallel. In the initial period of the anti-apoptosis mechanisms prevail, and in later periods dominated phenomena pro-apoptosis. PARP-1 activation indicates an increase in the frequency of DNA damage cells, and the duration of this process stimulates cell death by caspase-independent pathway. Together, these processes result in the elimination of a large number of cells, especially fibroblast of zone connective tissue formation in an aseptic area of inflammation in the peritoneal injury. We first established that in the case of peritoneal injury marker expression of anti-apoptosis in the damage zone has the character of the two-wave with maximum expression at 1-third day of the pathological process with repetitive peak on the 14th day. Identifying key parts of apoptosis in the formation of adhesions in the abdominal cavity, which can be used for the development of drugs for the prevention of adhesions.