Expression of acylamidase gene in Rhodococcus erythropolis strains
Lavrov, K.V.; Novikov, A.D.; Riabchenko, L.E.; Ianenko, A.S.
Genetika 50(9): 1133-1137
2014
ISSN/ISBN: 0016-6758 PMID: 25735145 Document Number: 673051
The expression of a new acylamidase gene from R. erythropolis 37 was studied in Rhodococcus erythropolis strains. This acylamidase, as a result of its unique substrate specificity, can hydrolyse N-substituted amides (4'-nitroacetanilide, N-isopropylacrylamide, N'N-dimethylaminopropylacrylamide). A new expression system based on the use of the promoter region of nitrilhydratase genes from R. rhodochrous M8 was created to achieve constitutive synthesis of acylamidase in R. erythropolis cells. A fourfold improvement in the acylamidase activity of recombinant R. erythropolis cells as compared with the parent wild-type strain was obtained through the use of the new expression system.