Wuziyanzong compound relieves oxidative stress injury and inhibits the apoptosis of Sertoli cells
Yin, J.-L.; Xu, Y.; Wu, B.
Zhonghua Nan Ke Xue 19(3): 257-261
2013
ISSN/ISBN: 1009-3591 PMID: 23700734 Document Number: 670989
To investigate the effects of Wuziyanzong Compound on oxidative stress injury and the apoptosis of Sertoli cells. We added H2O2 (100 mmol/L) and Wuziyanzong extract to the culture solution of TM4 cells, which were divided into a control, a model and three Wuziyanzong groups (low dose: 0.2 mg/ml, medium dose: 1 mg/ml, and high dose: 5 mg/ml). After 24 hours of intervention, we detected the absorbance value by MTT assay, measured the SOD activity and MDA content using the xanthine oxidase and thiobarbituric acid method respectively, and determined the apoptosis and survival rate of the cells by flow cytometry and the expression of caspase-3 mRNA by real time PCR. After treated with low-, medium- and high-dose Wuziyanzong Compound, the absorbance value of the TM4 cells was increased from 0.23 +/- 0.01 in the model group to 0.27 +/- 0.02, 0.30 +/- 0.01 and 0.35 +/- 0.01, the SOD activity increased from (8.32 +/- 0.62) U/ml to (9.69 +/- 0.59), (10.47 +/- 0.74) and (12.28 +/- 0.38) U/ ml, the MDA content decreased from (6.99 +/- 0.74) mol/L to (5.78 +/- 0.28), (4.19 +/- 0.43) and (3.42 +/- 0.51) mol/L, the cell apoptosis rate decreased from (21.63 +/- 1.38)% to (17.87 +/- 0.75), (14.10 +/- 0.50) and (9.53 +/- 1.34)%, the cell survival rate increased from (65.67 +/- 7.48)% to (70.37 +/- 1.12), (72.57 +/- 1.95) and (81.60 +/- 2.46)%, and the relative expression of caspase-3 mRNA decreased from 2.69 +/- 0.16 to 2.47 +/- 0.12, 2.38 +/- 0.16 and 1.96 +/- 0.11, respectively. Wuziyanzong Compound can relieve oxidative stress injury and inhibit the apoptosis of TM4 cells.