Molecular identification of raw materials from lian qiao bai du wan
Cui, Z.-H.; Jiang, C.; Li, M.-H.; Chen, M.; Zhou, L.-S.; Yuan, Y.
Yao Xue Xue Bao 48(4): 590-596
2013
ISSN/ISBN: 0513-4870 PMID: 23833950 Document Number: 670920
Lian Qiao Bai Du Wan was used to study the identification of Chinese patent medicine by molecular marker technique. DNA was extracted through modified CTAB method. The psbA-trnH and rbcL sequences were gradient amplified, and PCR products were ligated with the pEASY-T5 vector and then transformed into Trans1-T1 cells, respectively. Clones were selected randomly and sequenced. All sequences were analyzed by BlastN and the neighbor-joining (NJ) phylogenetic tree was constructed by MEGA 4.0. The results showed that nine kinds of medicinal materials can be identified by psbA-trnH sequences, and six kinds of medicinal materials by rbcL sequences from Lian Qiao Bai Du Wan. Molecular marker technique can stably and accurately distinguish multi-origin medicinal materials in Chinese patent medicine.