Effect of RelB on HIV-1 Vpr-mediated transcription activation and cell G2/M arrest
Liu, R.-K.; Gao, Y.; Lin, Y.-Q.; Tan, J.; Geng, Y.-Q.; Qiao, W.-T.
Bing du Xue Bao 29(1): 44-50
2013
ISSN/ISBN: 1000-8721 PMID: 23547379 Document Number: 668239
Vpr, an auxiliary protein of HIV-1(Human immunodeficiency virus type 1), exerts important functions to promote viral replication and AIDS progression. In this study, we performed a yeast two-hybrid screening assay using human cDNA library to further investigate the molecular mechanism of various functions of Vpr RelB, a key protein in NF-kappaB signaling pathway, was identified as a Vpr interaction protein by co-immunoprecipitation. Further investigations indicated that RelB not only promoted the Vpr-mediated activation of NF-kappaB reporter gene, but also enhanced the transactivation of HIV LTR. Moreover, the results showed that RelB promoted Vpr-induced cell cycle G2/M arrest. Collectively, these results indicated that RelB might interact with Vpr and regulate its transcriptional activation and cell cycle arrest.