Radioimmunoassay of rat 1 -fetoprotein
Sell, S.
Cancer Research 33(5): 1010-1015
1973
ISSN/ISBN: 0008-5472 PMID: 4122163 Document Number: 66486
A competitive binding radioimmunoassay for rat alpha 1 fetoprotein (AFP) was developed, using Sprague-Dawley rat amniotic fluid. The assay was approximately 20,000 times more sensitive than double-diffusion in agar for AFP detection; the assay threshold was 5 ng. Further purification of apparently pure (by immunodiffusion and immunization) radiolabeled AFP was required for the specific assay. An assay for a previously undetected contaminant(s) was used to check the purity of rat AFP isolated by isoelectric focusing to obtain the purified unlabeled AFP needed to establish the standard inhibition curve. All procedures are outlined.