Development of biochip for determination of Y-haplogroups occuring in Russian populations
Fesenko, D.O.; Kalennik, O.V.; Barskiĭ, V.E.; Zasedatelev, A.S.; Nasedkina, T.V.
Molekuliarnaia Biologiia 46(5): 814-818
2012
ISSN/ISBN: 0026-8984 PMID: 23156682 Document Number: 663687
Biochip has been developed which allowed to determine the following Y-chromosome haplogroups: C, DE, G, H, I, J, L, N, O, R in a DNA sample. The following SNPs were choosen as haplogroup markers: M130, M145, P257, M69, U179, M304, M185, M231, M175, P224, correspondingly. The genotyping included two-round PCR with fluorescent label incorporation into PCR product followed by hybridization with immobilized probes on biochip. The analysis of fluorescent signal ratios in pairs of immobilized probes "wild-type probe"--"group specific probe" for each of choosen polymorphic markers showed high accuracy of Y-haplogroup genotyping using biochip. The reliability of genotyping was confirmed by direct sequencing.