The use of hydrosol hexacianferrate (II) ferrum (III) for developing diagnostic lateral flow tests
Skopinskaia, S.N.; Iarkov, S.P.; Zlobin, V.N.; Valiev, K.Kh.
Vestnik Rossiiskoi Akademii Meditsinskikh Nauk 2012(3): 80-85
2012
ISSN/ISBN: 0869-6047 PMID: 22712280 Document Number: 662277
On the basis of synthesized negatively charged hydrosol hexacianferrate (II) ferrum (III) (HCFF) by diameter 10-20 HM are received stable conjugates with antibodies and antigens glycoprotein and lipopolysaccharide (LPS) nature. Synthesized hydrosol (HCFF) is new type of a disperse phase in the lateral flow assay. Conjugates mentioned above were applied for construction lateral flow tests-systems for revealing cholera toxin, the rabbit antibodies to recombinant glycoproteine complex from Micobacterium tuberculosis H37 Rv, human immunoglobulin, LPS antigens S. typhimurium, S. enteritidis. Developed lateral flow tests-systems had high analysis speed (5-7 min), good specificity and sensitivity: on cholera toxin of 2.0 ug/ml, on LPS antigens S. typhimurium, S. enteritidis 0.5 ug/ml.