Effect of selenium on the protection of myocardial cells from injuries induced by overloaded reactive oxygen species, and on the expression of actin in myocardial cells
Tang, J.; Tan, W.; Zhu, Y.; Wang, L.; Zhai, L.
Wei Sheng Yan Jiu 41(1): 2-5; 12
2012
ISSN/ISBN: 1000-8020 PMID: 22443049 Document Number: 661528
To investigate the effect of selenium on the protection of myocardial cells from injuries induced by H2O2 and on the expression of alpha-actin and beta-actin in myocardial cells. Myocardial cells of suckling mice in the culture were divided into six groups: Controls group (without H2O2 or Se), H2O2 group, Se 0.05 micromol/ L group, Se 0.5 micromol/L group, Se 1.0 micromol/L group and Se 5.0 micromol/L group. The ultrastructure of myocardial cells was observed by transmission electron microscope (TEM), and the LDH and MDA contents in the culture media were determined by colorimetry. The expression of alpha-actin and beta-actin in myocardial cells was detected by Western blot. The injury of myocardial cells observed under TEM was attenuated in the 0.5 micromol/L Se group. The LDH and MDA contents in the culture media of the Se groups was higher than the control group (P < 0.05), but lower than the H2O2 group (P < 0.05). The LDH and MDA contents in the 0.5 micromol/L Se group were the lowest in all Se groups. The expression level of alpha-actin and beta-actin in the 0.5 micromol/L Se group is higher than that in the H2O2 group, even higher than the control group. The protective effect of Se on myocardial cells damaged by H2O2 was better in the 0.5 micromol/ LSe group, which could maintain the expression of alpha-actin and beta-actin, even induce the remolding of cytoskeleton proteins.