Metformin effect on urethane-induced tumorigenesis in mice
Popovich, I.G.; Piskunova, T.S.; Tyndyk, M.L.; Anikin, I.V.; Zabezhinskiĭ, M.A.; Anisimov, V.N.
Voprosy Onkologii 58(4): 549-553
2012
ISSN/ISBN: 0507-3758 PMID: 23607214 Document Number: 661250
Sixty one male 129/Sv mice were exposed to a single intraperitoneal injection of 1 g per kilo of urethane dissolved in 0.9% normal saline. Starting the next day from the injection the study group mice were given 1200 mg metformin per liter of drinking water 5 days a week for 26 weeks. The control group mice received pure drinking water. Six months after the urethane treatment the mice were killed and the morphology samples were taken. Twenty five of 31 (96.7%) control group mice developed tumors (lung adenomas and thymic lymphomas), while tumor development was observed in 25 of 31 (80.7%; p<0.05) mice exposed to metformin. Solid or trabecular lung adenomas developed in 90% of the control group mice and in 77% of the metformin group mice (p=0.119). Therefore, it is a first evidence of tumor-inhibitory effect of metformin in mice.