Use of dried blood spots for HIV-1 genotyping in Southeast Asia: Thailand experience
Rutvisuttinunt, W.; Arroyo, M.A.; Assawadarachai, V.; Poltavee, K.; McCutchan, F.E.; Tovanabutra, S.; Kijak, G.; Kim, J.H.; Paris, R.M.; de Souza, M.
Southeast Asian Journal of Tropical Medicine and Public Health 43(2): 333-339
2012
ISSN/ISBN: 0125-1562 PMID: 23082585 Document Number: 660184
The multi-region hybridization assay (MHAbce) for genotyping HIV-1 subtypes B, C and circulating recombinant form (CRF01_AE) was evaluated on paired plasma and dried blood spots (DBS) collected from 68 HIV-1 infected individuals in Thailand. CRF01_AE was the predominant subtype identified using plasma samples (51/62) and DBS (24/27). There was no discordance in subtype designations between plasma and DBS.