Study on electrophoretogram of Magnolia officinalis seeds from different locations
Li, X.; Shao, A.; Chen, M.; Huang, L.
Zhongguo Zhong Yao Za Zhi 36(22): 3075-3078
2011
ISSN/ISBN: 1001-5302 PMID: 22375381 Document Number: 655539
To find the identification method of seeds from different locations, we analyzed the SDS-PAGE electrophoretogram of Magnolia officinalis seeds from 5 different locations of Enshi district, Hubei province, the genuine producing area of M. officinalis, and compared band numbers, band intensity and protein molecular weight of different locations. The result showed that there were significant differences among seeds from different locations. 9 bands with the molecular weight about 80.34, 63.07, 56.48, 51.32, 40.64, 25.18, 22.63, 21.70, 18.14 kDa were involved in all seeds electrophoretograms and could be as standard bands of M. officinalis seeds. Seeds from Badong county, Jianshi county and Enshi city had their own special bands which were very different from other locations. So this electrophresis method can be applied to identify different locations of M. officinalis seeds.