Effects of EMPs on HUVECs' functions in vitro
Zheng, M.-Z.; Wu, Z.-H.; Ji, C.-L.; Li, H.; Qiu, G.-X.; Weng, X.-S.
Zhonghua Yi Xue Za Zhi 91(5): 337-339
2011
ISSN/ISBN: 0376-2491 PMID: 21419011 Document Number: 653421
To investigate the effects of EMPs on HUVECs permeability in vitro, then evaluate the effect on the Avascular Necrosis of Femoral Head. Incubate HUVECs with EMPs which is released by HUVECs in vitro, then estimate the endothelial permeability. Flow cytometry analysis showed that EMPs were released at maximum concentration(7.66 × 10(4)/ml) after 24 hours stimulated by 10 mmol DEX And 7.66 × 10(4), 7.66 × 10(3)/ml concentration (moderate and high concentrations) of EMPs caused injury to the permeability of HUVECs (P values are 0.002 and 0.031 respectively, < 0.05), while 7.66 × 10(2)/ml concentration (low concentration) of EMPs didn't (P = 0.425 > 0.05). Dexamethasone can induce HUVECs to release EMPs in vitro. EMPs caused collapse of connections of HUVECs, and increase the HUVECs permeability, decrease vascular stability, finally lead to Avascular Necrosis of Femoral Head.