Identification of major allergens of wildflower honey
Yadzir, Z.Hani.Mohamad.; Misnan, R.; Abdullah, N.; Arip, M.; Murad, S.
Southeast Asian Journal of Tropical Medicine and Public Health 42(2): 370-375
2011
ISSN/ISBN: 0125-1562 PMID: 21710860 Document Number: 652368
The aim of this study was to identify the major allergens of wildflower honey in local patients with atopic disease. SDS-PAGE revealed ten protein bands of 25 to 110 kDa, with a heavy cluster in region of 40-75 kDa. Immunoblotting demonstrated seven IgE-binding bands of 39 to 110 kDa. The 60 kDa protein had the highest frequency of IgE-binding (100%) followed by 54 kDa protein (95%), thus identified as the major allergens of wildflowerhoney. Our findings indicate that the allergen extract used for diagnosis of honey allergy contains both the 54 kDa and 60 kDa proteins.