The establishment of real time fluorescent PCR detecting method and preliminary application on pathogenesis of HFMD
Tang, Y.; Hou, J.; Xu, J.; Sheng, H.-h.; Chen, W.-w.; Li, B.-a.; Zhao, J.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 25(2): 152-154
2011
ISSN/ISBN: 1003-9279 PMID: 21863646 Document Number: 652111
To establish the real-time fluorescent PCR method for detecting enterovirus, enterovirus 71 and Coxsackievirus A 16 nucleic acid. Primers and MGB probe were chosen for virus gene. The samples of 38 HFMD patients were analyzed by TaqMan-MGB PCR technique on a fluorescence real-time PCR instrument,and the results were compared with those by conventional RT-PCR. The real-time fluorescent PCR positive rates of EV, EV71 and Cox A16 were 73.7%, 60.5%, 13.2%; the conventional RT-PCR were 71.1%, 55.3%, 13.2%. There were no significant differences between the two methods. The real-time fluorescent PCR detecting method of EV, EV71 and Cox A16 nucleic acid have been established successfully.