PDE4 inhibitor suppresses PGE2-induced osteoclast formation via COX-2-mediated p27 (KIP1) expression in RAW264.7 cells
Chen, L.; Zheng, T.; Park, H.; Noh, A.L.S.M.; Lee, J.-M.; Lee, D.-S.; Yim, M.
Die Pharmazie 66(3): 201-206
2011
ISSN/ISBN: 0031-7144 PMID: 21553651 Document Number: 649371
We investigated the effects of phosphodiesterase 3 (PDE3) and PDE4 inhibitors, which are cAMP degrading enzymes, on prostaglandin E2 (PGE2)-induced osteoclast formation. A PDE4 inhibitor decreased PGE2-induced osteoclast formation, whereas a PDE3 inhibitor did not, possibly due to the lack of PDE3 expression in RAW 264.7 cells. Cell cycle analysis revealed that the PDE4 inhibitor stimulated PGE2-induced p27(KIP1) expression, which leads to increased growth arrest at G0/G1 phase. The PDE4 inhibitor increased cyclooxygenase 2 (COX-2) expression in the presence of PGE2. COX-2 overexpression was associated with growth suppression via p27(KIP1) expression in RAW 264.7 cells. Taken together, our data demonstrate that the PDE4 inhibitor enhances PGE2-induced growth arrest of osteoclast precursors via COX-2-mediated p27(KIP1) expression, which in turn negatively regulates osteoclast formation.