Quality evaluation of artificial musk based on its inhibitory effect on cyclooxygenase-2
Luo, Y.; Jin, C.; Zhou, J.; Wen, R.-q.; Li, X.-f.; Li, R.-s.; Yang, M.; Xiao, X.-h.
Yao Xue Xue Bao 46(4): 438-442
2011
ISSN/ISBN: 0513-4870 PMID: 21751497 Document Number: 649185
The inhibitory ratio (1%) of artificial musk on cyclooxygenase-2 (COX-2) was determined by enzyme immunoassay (EIA). The dose-effect relationship between concentrations of artificial musk and 1% was established. It was found that artificial musk had obvious inhibitory action on COX-2. The concentration for 50% of maximum inhibitory effect (IC50) was about 2.26 mg x mL(-1). There was a good relationship between the logarithm concentrations of artificial musk and 1% when the concentrations of artificial musk ranged from 0.31-20.0 mg x mL(-1). The results indicated that this EIA method could be applied to evaluate the anti-inflammatory activity of artificial musk quickly, conveniently, sensitively and exactly. This paper provided a novel method and foundational research for the bioassay of artificial musk.