The establishment and evaluation of diagnostic accuracy of AllGlo (TM) probe-based techniques for invasive aspergillosis
Wu, D.-S.; Shen, J.-Z.; Zhou, X.-Q.; Shen, S.-F.; Wu, X.-M.
Zhonghua Nei Ke Za Zhi 49(2): 142-145
2010
ISSN/ISBN: 0578-1426 PMID: 20356513 Document Number: 647370
To establish and evaluate a diagnostic technique based on the AllGlo(TM) probe for the invasive aspergillosis. With the self-designed AllGlo(TM) probes and primers and the standards, two standard curves of the real-time PCR based on AllGlo(TM) probes were established respectively for A. flavus and A. fumigatus, then its specificity, sensitivity and reproducibility were evaluated respectively. The findings indicated that the standard curve of A. flavus was Y = -3.003X + 36.825, and A.fumigatus' was Y = -3.052X + 38.016, and their interassay coefficient of variation respectively were 15.60% and 12.94%, suggesting a good reproducibility. The lowest spore concentration they could be detected was 10 CFU/ml, which equated to 100 - 1000 copies of internal transcribed spacer (ITS)2 genes, suggesting a good sensibility. They didn't have cross-positive reaction with other fungus, human genome and bacteria, which indicated a good specificity. The diagnostic technique based on the AllGlo(TM) probe for the invasive aspergillosis possessed a good sensitivity, good specificity and deadly accuracy.