Effects of retinoic acid receptor agonist and retinoid X receptor agonist on the expression of IL-4 and IFN-gamma in cord blood mononuclear cells
Zhu, D.; Wang, L.; Li, Y.; Zhu, Y.; Zhao, P.
Wei Sheng Yan Jiu 39(4): 433-435
2010
ISSN/ISBN: 1000-8020 PMID: 20726231 Document Number: 647261
To observe the effects of retinoic acid receptors agonist TTNPB and retinoid X receptors agonist MA on both protein and gene expression of IFN-gamma and IL-4 in cultured cord blood mononuclear cells. Observing both the protein content and gene expression of IFN-gamma and IL-4 with ELISA and fluorescent real-time quantitative PCR in vitro. It was indicated by ELISA that 10(-8), 10(-6), 10(-5) and 10(-4) mol/L TTNPB and 10(-8), 10(-5) and 10(-4) mol/L MA decreased the ratio of IFN-gamma/IL-4 significantly in comparison with the control (P < 0.05), and indicated by RT-PCR that 10(-4) mol/L MA decreased the ratio of IFN-gamma/IL-4mRNA significantly and 10(-5) mol/L TTNPB and 10(-7), 10(-6) mol/L MA increased the ratio of IFN-gamma/IL-4mRNA significantly in comparison with the control (P < 0.05). The ratio of IFN-gamma/IL-4 could be down-regulated by proper concentration of TTNPB and MA at the protein level, while the ratio of IFN-gamma/IL-4mRNA was differently influenced by various concentration of TTNPB and MA at the gene level.