Study on IgG promoter as probe to evaluate safety of injections in pre-clinic
Wang, Y.; Li, L.; Lei, F.; Liang, A.; Xing, D.; Du, L.
Zhongguo Zhong Yao Za Zhi 35(1): 76-79
2010
ISSN/ISBN: 1001-5302 PMID: 20349721 Document Number: 646758
To construct an effective system screening and evaluating possible injections and components inducing allergy type II. Transfect IgG promoter-regulated green fluorescent protein expressing plasmid into RPMI-8226 cell. The number of fluorescent cells after drug treatment was calculated and statistically evaluated. Tween 80 can suppress the expression of IgG effectively, and puerarin has activity of stimulating IgG expression, and the system has no response to KCl-treatment. No effect of Yuxingcao (Herba Houttuyniae) injection on this system is observed. IgG promoter-drove green fluorescent protein expressing cell line can be used as system screening injections and components inducing allergy type II based on stimulating IgG promoter activity. Tween 80 can suppress the expression of IgG at the transcriptional level. Four batches of Yuxingcao injection cannot induce allergy type II by activating IgG expression.