Comparative evaluation of PCR in Ziehl-Neelsen stained smears and PCR in tissues for diagnosis of Mycobacterium avium subsp. paratuberculosis
Coelho, A.C.; Pinto, M.L.; Miranda, A.; Coelho, A.M.; Pires, M.A.; Matos, M.
Indian Journal of Experimental Biology 48(9): 948-950
2010
ISSN/ISBN: 0019-5189 PMID: 21506505 Document Number: 646322
Thirty six tissues from sheep, previously diagnosed with paratuberculosis, were tested by PCR in positive Ziehl-Neelsen staining smears of tissues, and PCR in tissues targeting IS900 specific for Mycobacterium avium subsp. paratuberculosis. DNA amplification was achieved in 33.3% Ziehl-Neelsen smears, and in 61.1% tissue samples. Combination of both techniques found 66.7% samples as positive. Combination of techniques would, therefore, increase the sensitivity of diagnosis.