Development and validation of HPLC-UV-MS method for the control of four anti-diabetic drugs in suspected counterfeit products
Dai, X.-m.; An, N.; Wu, J.-m.; Li, H.-y.; Zhang, Q.-m.
Yao Xue Xue Bao 45(3): 347-352
2010
ISSN/ISBN: 0513-4870 PMID: 21348424 Document Number: 643253
An HPLC-UV method has been developed for the determination of valibose, miglitol, voglibose and acarbose, the four anti-diabetic drugs. The separation was accomplished successfully by using reversed phase chromatography (Prevail carbohydrate column, 250 mm x 4.6 mm, 5 microm) with a gradient acetonitrile-phosphate buffer solution (pH 8.0) at a wavelength of 210 nm. Furthermore, the method of a high-performance liquid chromatography coupled with ESI-MS in positive ionization mode has been established. These two methods were successfully applied to the assay and qualitative detection of four alpha-glucosidase inhibitors in the potential counterfeit anti-diabetic drugs.