Monitoring for influenza in population of Western Siberia in 2007-2009
Il'icheva, T.N.; Romanovskaia, A.A.; Il'inykh, F.A.; Kurskaia, O.G.; Durymanov, A.G.; Ignashkina, M.B.; Kulak, M.V.; Ivanova, E.V.; Bespalov, V.S.; Gorbatovskaia, L.M.; Sivaĭ, M.V.; Shestopalov, A.M.; Malkova, E.M.; Drozdov, I.G.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 2010(6): 26-30
2010
ISSN/ISBN: 0372-9311 PMID: 21381373 Document Number: 643078
To analyze influenza viruses isolated in the 2008-2009 autumn-winter season, and to test sera collected in the south of Western Siberia during the beginning and the end of the epidemic seasons from 2007 until the A/H1N1 pandemic. Total 149 clinical samples were analyzed and 2190 blood sera were tested. During the 2008-2009 season 17 influenza viruses were isolated. 9 of these were A/H1N1, 5-were A/H3N2, and 3 were influenza B viruses. The nucleotide sequences and amino acid composition of influenza A virus hemagglutinin (HA) were compared with reference strains. Among A/H1N1 viruses circulating in Novosibirsk region three viruses contained four amino acid replacements in antigen sites Ca, Cb and Sb. In A/ H3N2 viruses from Novosibirsk, 2 amino acid substitutions were detected in antigen sites B and E. Based on genotyping influenzae epidemic on February to April of 2009 in the south of western Siberia was associated with influenza viruses A/H1N1, A/H3N2, and B. All A/H3N2 influenza virus isolates were variants of reference A/Brisbane/10/2007(H3N2) and A/ H1N1 influenza viruses isolates were similar to reference A/Brisbane/59/2007(H1N1).