On-line sample stacking for the analysis of glycyrrhiza flavonoids by anion selective exhaustive injection-sweeping micellar electrokinetic chromatography
Chen, X.; Yuan, H.; Cao, Y.; Chen, Q.
Se Pu 28(9): 889-892
2010
ISSN/ISBN: 1000-8713 PMID: 21171288 Document Number: 642327
An on-line preconcentration technique which coupled anion selective exhaustive injection (ASEI)-sweeping technology with micellar electrokinetic chromatography (MEKC) was used to analyze three glycyrrhiza flavonoids (isoliquiritigenin, liquiritigenin and liquiritin) in medicine. The separation conditions were optimized in conventional MEKC. The sample matrix, injection time of water and injection time of sample were found to be the predominant influences on the glycyrrhiza flavonoids stacking. Under optimal conditions, the detection sensitivities were improved by 110, 120, 300 times for isoliquiritigenin, liquiritigenin and liquiritin, respectively. The limits of detection (LODs) were 0.015, 0.014, 0.011 mg/L for isoliquiritigenin, liquiritigenin and liquiritin, respectively. The recoveries were ranged in 90.6%-107% and relative standard deviations (RSDs) were less than 4.5% (n=3). This method was successfully applied for the detection of isoliquiritigenin, liquiritigenin and liquiritin in real samples.