Novel microbial route to synthesize silver nanoparticles using spore crystal mixture of Bacillus thuringiensis
Jain, D.; Kachhwaha, S.; Jain, R.; Srivastava, G.; Kothari, S.L.
Indian Journal of Experimental Biology 48(11): 1152-1156
2010
ISSN/ISBN: 0019-5189 PMID: 21117457 Document Number: 642069
Metallic nanoparticles are traditionally synthesized by wet chemical techniques, where the chemicals used are often toxic and flammable. In the present study, the spore crystal mixture of Bacillus thuringiensis was used for the synthesis of silver nanoparticles. Nanoparticles were characterized using UV-Vis absorption spectroscopy, XRD and TEM. X-ray diffraction and TEM analysis showed the average particle size of 15 nm and mixed (cubic and hexagonal) structure. This is for the first time that any bacterial spore crystal mixture was used for the synthesis of nanoparticles. Further, these biologically synthesized nanoparticles were found to be highly toxic against different multi drug resistant human pathogenic bacteria.