Evaluating of influence of repeated thaw/freeze cycles on IgG and IgM stability
Siennicka, J.; Laskowska, A.; Trzcińska, A.
Medycyna Doswiadczalna i Mikrobiologia 62(3): 281-283
2010
ISSN/ISBN: 0025-8601 PMID: 21114021 Document Number: 640856
The purpose of this study was evaluating influence of repeated cycles of thawing and freezing serum samples on IgG and IgM stability. Serum positive samples for anti-cytomegalovirus and antimeasles virus IgG and/or IgM were aliquoted. Tubes containing 100 microl aliquot were frozen at -20 degrees C. Samples were repetitively thawed and froze in one to ten cycles. Antibodies presence was examined with commercially available ELISA tests. All samples reminded positive even after ten repeated thaw/freeze cycles.