Immunostimulatory effects of extract of Pulicaria crispa before and after Schistosoma mansoni infection
Maghraby, A.S.; Shalaby, N.; Abd-Alla, H.I.; Ahmed, S.A.; Khaled, H.M.; Bahgat, M.M.
Acta Poloniae Pharmaceutica 67(1): 75-79
2010
ISSN/ISBN: 0001-6837 PMID: 20210082 Document Number: 639478
The immunostimulatory effects of methanolic extract from Pulicaria crispa were investigated in mice before and after infection with Schistosoma mansoni. Mice were subjected for daily intra-peritoneal injection by the extract (33 ng/mouse) for 10 successive days followed by infecting every mouse with 100 S. mansoni cercariae. Treatment with the extract induced significant increase (p < 0.05) in sera-IL-2 before and after infection. Upon using soluble worm antigen preparation or cancer bladder homogenates as antigens in ELISA, the detected levels of IgG were significantly (p < 0.05) higher in sera from treated-infected mice than untreated P. crispa infected mice. Using crude Escherichia coli lysate as an antigen in ELISA, it was detected a significant (p < 0.05) increase in IgG levels in sera from the extract-treated mice before and after infection.