Purification of the mitochondrial calcium uniporter from the beef heart and characterization of its properties
Gritsenko, E.N.; Kutyshenko, V.P.; Saris, N.E.-L.; Wahlsten, M.; Jokela, J.; Mironova, G.D.
Biofizika 55(5): 803-808
2010
ISSN/ISBN: 0006-3029 PMID: 21033345 Document Number: 639072
A low-molecular-weight component (LMC) inducing selective transport of calcium across the bilayer lipid membrane has been isolated from mitochondria of the bovine heart by the method developed in our laboratory, which excludes the use of detergents and proteolytic enzymes. It was shown that, in the presence of 10 mM CaCl2, LMC forms conduction channels in the membrane multiples of 5 pS. The specific inhibitor of mitochondrial calcium uniporter, ruthenium red, closes Ca2(+)-induced channels formed in the membrane by LMC. In the absence of calcium or in the presence of potassium ions only, the component is incapable of forming channels of conduction. It was shown using nuclear magnetic resonance that LMC is a complex consisting of lipids, amino acids, and sugars with a molecular weight of 1-2 kDa.