Isolation of genomic DNA from medicinal plants without liquid nitrogen
Sharma, P.; Joshi, N.; Sharma, A.
Indian Journal of Experimental Biology 48(6): 610-614
2010
ISSN/ISBN: 0019-5189 PMID: 20882764 Document Number: 638652
Genomic DNA was extracted from eight medicinal plants using the present DNA extraction protocols (CTAB extraction method) with some modifications. Leaves were fixed in different fixing solutions containing absolute alcohol (99.99%), chloroform and EDTA, but without liquid nitrogen. DNA quality and quantity obtained were comparable to those isolated with liquid nitrogen, as the lambda260/lambda280 ratio with liquid nitrogen was in range 1.3-1.7 and with other fixing solutions it was 1.1-1.5. Absolute alcohol showed best results as fixing solution. Good quality of DNA was isolated without using liquid nitrogen from different medicinal plant species. DNA isolated by this method was suitable for various molecular biology applications.