Microleakage of human saliva in coronally unsealed obturated root canals in anaerobic conditions
Yazdi, K.A.; Bayat-Movahed, S.; Aligholi, M.; Hayes, S.J.; Nekoofar, M.H.
Journal of the California Dental Association 37(1): 33-37
2009
ISSN/ISBN: 1043-2256 PMID: 19263626 Document Number: 635658
The purpose of this study was to determine the time required for anaerobic bacteria in natural human saliva to contaminate root-filled teeth. Thirty-two single-rooted teeth were cleaned, shaped, filled, and exposed to human saliva for 120 days. Teeth that had not leaked were subjected to polymerise chain reaction examination. Sixty-six percent of the experimental group were totally contaminated. A PCR examination revealed there was no contamination in the apical 3 mm of leakage-free teeth.