Isolation, identification and diversity analysis of petroleum-degrading bacteria in Shengli Oil Field wetland soil
Han, P.; Zheng, L.; Cui, Z.-S.; Guo, X.-C.; Tian, L.
Ying Yong Sheng Tai Xue Bao 20(5): 1202-1208
2009
ISSN/ISBN: 1001-9332 PMID: 19803182 Document Number: 635214
The petroleum-degrading bacteria in Shengli Oil Field wetland soil were isolated and identified by traditional experiment methods, and their diversity was analyzed by PCR-DGGE (denaturing gradient gel electrophoresis). A total of thirteen petroleum-degrading bacterial strains were isolated, among which, six strains were found to have the ability of degrading the majority of C12-C26 petroleum hydrocarbon, with a degradation rate of > 90%. These petroleum degraders were phylogeneticly identified as the members of Halomonas, Alcanivorax, and Marinobacter, which were all belonged to gamma-proteobacteria. The uncultured predominant bacteria in Shengli Oil Field wetland soil were of Sulfurovum, Gillisia and Arcobacter. Among the predominant bacteria, gamma-proteobacteria accounted for a larger proportion, followed by alpha-proteobactiria, epsilon-proteobactiria, Actinobacteria, and Flavobacteria.