Trisomy of chromosome 8 in Ph-negative cells of the bone marrow in patients with chronic myeloid leukemia treated with inhibitors of BCR-ABL tyrosine kinases
Turkina, A.G.; Domracheva, E.V.; Vorontsova, A.V.; Aseeva, E.A.; Vinogradova, O.I.; Stakhina, O.V.; Gusarova, G.A.; Diagileva, O.A.; Semenova, E.A.; Vakhrusheva, M.V.; Kolosheĭnova, T.I.; Abakumov, E.M.; Chelysheva, E.I.; Goriacheva, S.R.; Ivanova, T.V.; Zakharova, E.S.; Kolosova, L.I.; Zakharova, A.V.; Naumova, I.N.; Diachenko, L.V.; Kulikov, S.M.; Kovaleva, L.G.; Khoroshko, N.D.
Terapevticheskii Arkhiv 81(7): 29-36
2009
ISSN/ISBN: 0040-3660 PMID: 19708570 Document Number: 632298
To analyse clinical implications of chromosome 8 trisomy in Ph-negative cells of the bone marrow in patients with chronic myeloid leukemia (CML) treated with inhibitors of tyrosinkinases (ITK). A total of 386 patients with CML (chronic phase--288, acceleration phase--77) received imatinib (400-800 mg/day). Because of resistance and/or intolerance some patients were switched to ITK II (nilotinib, dasatinib, bozutinib). This study included 8 CML patients (7 in a chronic phase, 1 in acceleration phase) treated with BCR-ABL ITK inhibitors of the first (imatinib) and the second line (ITK-II). The standard cytogenetic examination, on demand--investigation of the interphase nuclei with FISH, in some cases morphological, cytochemical and histological examinations of the bone marrow were made. The existence of a Ph-negative clone with trisomy of chromosome 8 had no negative effect on the course of the disease. The patients showed a stable hematological and cytogenetic response and no need in changing treatment policy. In long-term follow-up Ph-negative clone with trisomy of the chromosome 8 persisted without a clear trend to rise in most patients. Detection of a Ph-negative clone with chromosome 8 trisomy at early stages suggests parallel existence of Ph-positive and Ph-negative clones. None of the patients had myelodisplasia.