Amelogenin expression in cultured human dental pulp and periodontium related cells
Li, X.-t.; Shu, R.; Wen, H.-y.; Cheng, L.; Jiang, S.-y.
Shanghai Kou Qiang Yi Xue 18(5): 499-504
2009
ISSN/ISBN: 1006-7248 PMID: 19907857 Document Number: 631623
To investigate gene expression of amelogenin (Am) in human gingival epithelial cells(HGEC) and also other oral ectomesenchyme cells (human gingival fibroblasts, human periodontal ligament fibroblasts and human pulp cells). The amelogenin mRNA expression patterns were determined by the reverse transcription polymerase chain reaction (RT-PCR),and the protein expression was studied with Western blotting. There was no amelogenin expression detected in any of the cells. These findings suggest that amelogenin expression could not be detected in cultured human periodontium-related cells. Supported by National Natural Science Foundation of China (Grant No.30672315) and Research Fund of Science and Technology Commission of Shanghai Municipality(Grant No.08DZ2271100).