The effect of coaggregation of A.viscosus and P.gingivalis on the adherence of A.viscosus to hydroxyapatite
Miao, Y.; Chu, J.-P.; Wang, H.-F.; Lou, H.; Hao, Y.-Q.
Shanghai Kou Qiang Yi Xue 18(2): 203-206
2009
ISSN/ISBN: 1006-7248 PMID: 19418001 Document Number: 631587
To examine the effect of coaggregation of A.viscosus and P.gingivalis on the adherence of A.viscosus to saliva-coated hydroxyapatite(S-HA). The suspensions of A.viscosus and P.gingivalis were rotated and dropped on cover slips to visualize the coaggregation under scanning electron microscope(SEM). Different concentrations of P.gingivalis were mixed with A.viscosus radiolabeled with 10 uCi of [methyl-(3)H] thymedine per milliliter, then rotated for periods up to 1.5 hours at room temperature and the numbers of absorbed A.viscosus cells were determined by liquid scintillation. A.viscosus alone was used as the control, and its adhesion percent was set for 100%. One-way ANOVA with SPSS10.07 software package was used to analyze the effect of P.gingivalis on adherence of A.viscosus. Several cells of P.gingivalis could aggregate on the surface of A.viscosus. The adherence of A.viscosus to S-HA was promoted slightly when the concentration of P.gingivalis was rather small, then further increase in concentration of P.gingivalis resulted in a steady decline in the number of adherent A.viscosus, and the difference was significant (P<0.05). These results show that the adherence of A.viscosus to S-HA could be influenced by coaggregation of P.gingivalis and A.viscosus.