Influence of two Pt (IV) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors
Kaznica, A.; Drewa, T.; Lakomska, I.; Ryta-Stamirowska, P.; Debski, R.; Styczynski, J.; Drewa, G.; Szlyk, E.
Experimental Oncology 31(1): 33-36
2009
ISSN/ISBN: 1812-9269 PMID: 19300414 Document Number: 631557
Several platinum(IV) complexes are showing considerable promise in initial trials, producing reactive intermediates that then interact with DNA. To perform in vitro study of two new platinum(IV) complexes cytotoxic effect on B16 mouse melanoma cells. PtCl(4)(dbtp)(2) and PtCl(2)(6mp)(2) complexes were prepared. PtCl(4)(dbtp)(2) was created as modification of PtCl(4)(dmtp) test previously. Apoptosis and necrosis were examined using flow cytometry, upon Annexin V/PI staining. LC(10), LC(50) and LC(90) parameters established for PtCl(4)(dbtp)(2) were as following: 2.6, 17.0, 58.0 mumol/L. However LC(10) and LC(50) established for PtCl(2)(6mp)(2) were 1.2 and 14.0 micromol/l respectively. The both complexes induced apoptosis. PtCl(2)(6mp)(2) induced cell cycle arrest in G0/G1, while PtCl(4)(dbtp)(2) - in S-phase. PtCl(4)(dbtp)(2) appeared to be more cytotoxic against B16 cells than PtCl(2)(6mp)(2). Apoptosis was the main mechanism of cell loss in cultures incubated with both tested complexes.