Extract human DNA from maggot crop contents by phenol-chloroform method coupled with paramagnetic particle method
Wang, X.; Cai, J-Feng.; Zhong, M.; Li, J-Bo.; Zhang, J-Guo.; Lan, L-Mei.
Fa Yi Xue Za Zhi 25(6): 421-424
2009
ISSN/ISBN: 1004-5619 PMID: 20225616 Document Number: 631399
To establish an effective phenol-chloroform method coupled with paramagnetic particle method for human DNA extraction from maggot crop contents in STR genotyping. Human DNA was extracted from the maggot crop contents using phenol-chloroform method and purified by paramagnetic particle method. DNA was quantified by PCR with Quantifiler Human DNA Quantification Kit using 7500 real-time fluorescence quantitative PCR instrument. PCR products were genotyped by AmpFlSTR Identifiler PCR Amplification Kit using 3130XL-Avant genetic analyzer. The template DNA yield by the method described were increased at least 2 times than the phenol-chloroform extraction method alone. All of the full 16 STR profiles could be obtained with the samples extracted by this method when the DNA yield reached (0.218 +/- 0.041) ng/microL. Phenol-chloroform method coupled with paramagnetic particle method can effectively increase the sensitivity of STR analysis of human DNA recovered from maggot crop contents and is a valuable tool for forensic entomology.