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Role of three-color flow cytofluorometry in the algorithm for detection of minimal residual disease in children with acute lymphoblastic and acute myeloblastic leukemia

Savva, N.N.; Belevtsev, M.V.; Savitskiĭ, V.P.; Migal', N.V.; Movchan, L.V.; Aleĭnikova, O.V.

Klinicheskaia Laboratornaia Diagnostika 12: 15-18

2009

ISSN/ISBN: 0869-2084
PMID: 20140998
Document Number: 630947
Three-color flow cytofluorometry (FCF) was used to identify minimal residual disease in 124 patients with acute lymphoblastic leukemia (ALL) treated in accordance with the ALL-myeloblastic leukemia (MBL)-2002/2008 protocol and 36 patients with MBL treated under the MBL-MM-2000 protocol. It was shown that approximately a fifth of children with ALL, 3-color FCF could not monitor minimal residual disease, which required the use of 4- or more-color FCF and parallel determination in these MBL samples by molecular genetic methods. An algorithm of study of MBL by FCF, by additionally using molecular genetic methods, is presented.

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