Analysis of the variation of 32 HIV-1 V3 loop from infected Chinese blood/plasma donors
Dai, L.; Lu, J.-f.; Huang, X.-j.; Shi, Y.; Chen, D.-x.; Wu, H.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 22(1): 27-29
2008
ISSN/ISBN: 1003-9279 PMID: 18414693 Document Number: 628232
To characterize the variation in V3 loop of HIV-1 B'strains circulating in Chinese blood donors. The c2-c3 regions of the HIV envelop gene were amplified by nest-PCR from 32 HIV-1-infected blood donors in He Nan province in China. The BIOEDIT and MEGA software are used to analyze the sequences of V3 loop. There are five types of central motifs of the 32 samples, in which GPGR and GPGQ are most common. More variations associated with T tropic/SI phenotype can be seen in AIDS group. The V3 tip motifs of HIV-1B' strains circulating in Chinese blood donors are various, the different characterization of V3 loop between AIDS and asymptomatic patients indicates different biological phenotype and pathogenesis which warrant additional investigation.